This is an indoor capture. No flash was used. The subject was positioned in an angle across the window. The tripod was necessary cause I wanted to use a narrow aperture of f/16 to capture as much detail as possible. That let me with an exposure of 30 seconds in ISO 200.
Using Photoshop, I had to clean the spots of the flower that are visible only in 100% view when using a sharp lens as the Canon EF 100mm f/2.8L Macro IS USM.